Transfection is the “process of artificially introducing nucleic acids (DNA or RNA) into cells… using various chemical, biological, or physical methods” and “can result in a change of the properties of the cell”.1ThermoFisher.com. Archived link
Across its decades of use2e.g. Lipofectamine, an early transfection product, was launched in 1993; see also this 1989 paper. – as a laboratory tool for initiating changes that were only ever going to be studied in the short-term, repeated inquiries have been made into transfection’s inherently toxic effects.3e.g. in 1997 Basis of pulmonary toxicity associated with cationic lipid-mediated gene transfer to the mammalian lung (archived link); in 2000 Comprehensive analysis of the acute toxicities induced by systemic administration of cationic lipid:plasmid DNA complexes in mice (archived link); in 2003 Transfection efficiency and toxicity following delivery of naked plasmid DNA and cationic lipid–DNA complexes to ovine lung segments (archived link); in 2005 Toxicity of cationic lipid-DNA complexes (archived link); in 2007 Breaking up the correlation between efficacy and toxicity for nonviral gene delivery
(archived link); in 2012 Cellular Toxicity Caused by Transfection: Why is it important? (archived link); in 2014 Relating Toxicity to Transfection: Using Sphingosine To Maintain Prolonged Expression in Vitro (archived link); in 2019 Alkylation enhances biocompatibility and siRNA delivery efficiency of cationic curdlan nanoparticles (archived link); also 2019 Polymeric siRNA gene delivery – transfection efficiency versus cytotoxicity (archived link); in 2023 Improving cell and gene therapy safety and performance using next-generation Nanoplasmid vectors (archived link)
Which means:
- each COVID-19 mRNA injection is a transfection – not a “vaccine”
- and any side-effects are predictable outcomes of transfection – not “vaccine injuries”.
Sources
Videos
- 2024-01-19 with the Corona Investigative Committee
- 2024-02-20 with The Last American Vagabond
- 2024-08-22 with Rob Cunningham on The KUWL Show
- 2024-09-09 with Andrew For America on Episode 196 of The Politics And Punk Rock podcast
- 2024-09-24 Kayla Pollock with Dr. Jonathan Couey on The Lavigne Show
- 2024-10-01 with Medical Doctors For COVID Ethics International
- 2024-10-15 with Dr. Leyla Ali on Deprescribing #33
- 2024-10-17 Dear Senator Ron Johnson
- 2024-12-16 Monday Meddlers’ Medley
Screenshots
- 2022 University of Manitoba Gairdner Lecture by Dr. Pieter Cullis (timestamped)
- Cationic liposome-mediated RNA transfection. (1989, Robert Malone co-author)4Archived link: https://web.archive.org/web/20241003230610/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC297778/
- Couey, J. Jay profile page, University of Pittsburgh5Archived link: https://web.archive.org/web/20230327155000/https://neurobio-old.pitt.edu/faculty/couey.htm
- “Couey JJ” PubMed search6Archived link: https://web.archive.org/web/20230327155000/https://neurobio-old.pitt.edu/faculty/couey.htm
- Production of phagocytosis-inducing factor and expression of 4B4 antigen by cloned human T cells before and after transformation with HTLV-I (1988, Anthony Fauci co-author)7Archived link: https://archive.ph/tNZjH
- The Nobel Prize in Physiology or Medicine 20148Archived link: https://web.archive.org/web/20240927224020/https://www.nobelprize.org/prizes/medicine/2014/summary/
- ThermoFisher.com (Lipofectamine)9Archived links: (German) https://archive.ph/f9zJK, (English, 2023) https://web.archive.org/web/20230427115121/https://www.thermofisher.com/order/catalog/product/A12621
- ThermoFisher.com (Transfection Reagents)10Archived link: https://web.archive.org/web/20241231110144/https://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/transfection-reagents.html
- Virions released from cells transfected with a molecular clone of human T-cell leukemia virus type I give rise to primary and secondary infections of T cells (1995, Judy Mikovits co-author)11Archived link: https://archive.ph/iceor
- Yandex.com (‘gene gun’ image search)12could not archive
Footnotes
- 1
- 2e.g. Lipofectamine, an early transfection product, was launched in 1993; see also this 1989 paper.
- 3e.g. in 1997 Basis of pulmonary toxicity associated with cationic lipid-mediated gene transfer to the mammalian lung (archived link); in 2000 Comprehensive analysis of the acute toxicities induced by systemic administration of cationic lipid:plasmid DNA complexes in mice (archived link); in 2003 Transfection efficiency and toxicity following delivery of naked plasmid DNA and cationic lipid–DNA complexes to ovine lung segments (archived link); in 2005 Toxicity of cationic lipid-DNA complexes (archived link); in 2007 Breaking up the correlation between efficacy and toxicity for nonviral gene delivery
(archived link); in 2012 Cellular Toxicity Caused by Transfection: Why is it important? (archived link); in 2014 Relating Toxicity to Transfection: Using Sphingosine To Maintain Prolonged Expression in Vitro (archived link); in 2019 Alkylation enhances biocompatibility and siRNA delivery efficiency of cationic curdlan nanoparticles (archived link); also 2019 Polymeric siRNA gene delivery – transfection efficiency versus cytotoxicity (archived link); in 2023 Improving cell and gene therapy safety and performance using next-generation Nanoplasmid vectors (archived link) - 4
- 5
- 6
- 7Archived link: https://archive.ph/tNZjH
- 8
- 9Archived links: (German) https://archive.ph/f9zJK, (English, 2023) https://web.archive.org/web/20230427115121/https://www.thermofisher.com/order/catalog/product/A12621
- 10
- 11Archived link: https://archive.ph/iceor
- 12could not archive